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An assessment of retention behavior for gold nanorods in asymmetrical flow field-flow fractionation
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hadri, Hind El Gigault, Julien Tan, Jiaojie Hackley, Vincent A. |
| Copyright Year | 2018 |
| Abstract | AbstractApplications of asymmetrical flow field-flow fractionation (AF4) continue to expand rapidly in the fields of nanotechnology and biotechnology. In particular, AF4 has proven valuable for the separation and analysis of particles, biomolecular species (e.g., proteins, bacteria) and polymers (natural and synthetic), ranging in size from a few nanometers to several micrometers. The separation of non-spheroidal structures (e.g., rods, tubes, etc.) with primary dimensions in the nanometer regime, is a particularly challenging application deserving of greater study and consideration. The goal of the present study was to advance current understanding of the mechanism of separation of rod-like nano-objects in the AF4 channel. To achieve this, we have systematically investigated a series of commercially available cetyltrimethylammonium bromide stabilized gold nanorods (AuNRs), with aspect ratios from 1.7 to 10. Results show clearly that the retention time is principally dependent on the translational diffusion coefficient of the AuNRs. Equations used to calculate translational and rotational diffusion coefficients (cylinder and prolate ellipsoid models) yield similarly good fits to experimental data. Well characterized gold nanorods (length and diameter by transmission electron microscopy) can be used as calibrants for AF4 measurements allowing one to determine the aspect ratio of nanorod samples based on their retention times. Graphical abstractá…ź |
| Starting Page | 6977 |
| Ending Page | 6984 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://ws680.nist.gov/publication/get_pdf.cfm?pub_id=925959 |
| PubMed reference number | 30194453v1 |
| Alternate Webpage(s) | https://doi.org/10.1007/s00216-018-1325-y |
| DOI | 10.1007/s00216-018-1325-y |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume Number | 410 |
| Issue Number | 27 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | AF-4 protein AFF1 wt Allele Aspect Ratio Bromides CETRIMONIUM BROMIDE Coefficient Diameter (qualifier value) Fractionation, Field Flow Genetic Translation Process Nanorods Physical object Rod Photoreceptors Seizures Specimen Source Codes - Tube Transmission Electron Microscopy methscopolamine bromide |
| Content Type | Text |
| Resource Type | Article |
