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Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chang, Hyeyoon Oh, Seo-Eun Oh, Seunghan Hu, Kyunh-Seok Kim, Sungtae |
| Copyright Year | 2016 |
| Abstract | PURPOSE The aim of this study was to characterize the healing in the grafted calvarial defects of rats after adjunctive hyperbaric oxygen therapy. METHODS Twenty-eight male Sprague-Dawley rats (body weight, 250-300 g) were randomly divided into two treatment groups: with hyperbaric oxygen therapy (HBO; n=14) and without HBO (NHBO; n=14). Each group was further subdivided according to the bone substitute applied: biphasic calcium phosphate (BCP; n=7) and surface-modified BCP (mBCP; n=7). The mBCP comprised BCP coated with Escherichia-coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). Two symmetrical circular defects (6-mm diameter) were created in the right and left parietal bones of each animal. One defect was assigned as a control defect and received no bone substitute, while the other defect was filled with either BCP or mBCP. The animals were allowed to heal for 4 weeks, during which those in the HBO group underwent 5 sessions of HBO. At 4 weeks, the animals were sacrificed, and the defects were harvested for histologic and histomorphometric analysis. RESULTS Well-maintained space was found in the grafted groups. Woven bone connected to and away from the defect margin was formed. More angiogenesis was found with HBO and EGCG/BMP-2 (P<0.05). None of the defects achieved complete defect closure. Increased new bone formation with HBO or EGCG/BMP-2 was evident in histologic evaluation, but it did not reach statistical significance in histometric analysis. A synergic effect between HBO and EGCG/BMP-2 was not found. CONCLUSIONS Within the limitations of this study, the present findings indicate that adjunctive HBO and EGCG/BMP-2 could be beneficial for new bone formation in rat calvarial defects. |
| Starting Page | 244 |
| Ending Page | 253 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://synapse.koreamed.org/Synapse/Data/PDFData/1150JPIS/jpis-46-244.pdf |
| PubMed reference number | 27588214v1 |
| Alternate Webpage(s) | https://doi.org/10.5051/jpis.2016.46.4.244 |
| DOI | 10.5051/jpis.2016.46.4.244 |
| Journal | Journal of periodontal & implant science |
| Volume Number | 46 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adjunctive Orthodontic Procedure Bone Substitutes Bone neoplasms Closure Diameter (qualifier value) Fill Hyperbaric Oxygen (substance) Hyperbaric oxygenation therapy Neural Tube Defects Osteogenesis Oxygen Therapy Care P-Value Parietal bone structure PersonNameUse - assigned Recombinants Skeletal bone calcium phosphate epigallocatechin gallate hydroxyapatite-beta tricalcium phosphate |
| Content Type | Text |
| Resource Type | Article |
