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Optimization of simultaneous saccharification and fermentation for the production of ethanol from lignocellulosic biomass.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Krishna, S. Hari Chowdary, Ghanta Venkaiah |
| Copyright Year | 2000 |
| Abstract | Simultaneous saccharification and fermentation (SSF) of alkaline hydrogen peroxide pretreated Antigonum leptopus (Linn) leaves to ethanol was optimized using cellulase from Trichoderma reesei QM-9414 (Celluclast from Novo) and Saccharomyces cerevisiae NRRL-Y-132 cells. Response surface methodology (RSM) and a three-level four-variable design were employed to evaluate the effects of SSF process variables such as cellulase concentration (20-100 FPU/g of substrate), substrate concentration (5-15% w/v), incubation time (24-72 h), and temperature (35-45 degrees C) on ethanol production efficiency. Cellulase and substrate concentrations were found to be the most significant variables. The optimum conditions arrived at are as follows: cellulase = 100 FPU/g of substrate, substrate = 15% (w/v), incubation time = 57.2 h, and temperature = 38.5 degrees C. At these conditions, the predicted ethanol yield was 3.02% (w/v) and the actual experimental value was 3.0% (w/v). |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/jf991296z |
| PubMed reference number | 10820123 |
| Journal | Medline |
| Volume Number | 48 |
| Issue Number | 5 |
| Alternate Webpage(s) | http://www.people.iup.edu/jford/courses/CHEM421/Resources/OptimizationOfSimultaneousSaccharificationAndFermentationForTheProductionOfEthanolFromLignocellulosicBiomass.pdf |
| Alternate Webpage(s) | https://doi.org/10.1021/jf991296z |
| Journal | Journal of agricultural and food chemistry |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
