Modulation of crude glycerol fermentation by Clostridium pasteurianum DSM 525 towards the production of butanol

Content Provider	Semantic Scholar
Author	Gallardo, Ruber Alvès, Margot Rodrigues, Lígia R.
Copyright Year	2014
Abstract	Abstract High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clostridium pasteurianum DSM 525 is studied. Also, the effect of acetate and butyrate supplementation to the culture medium and the culture medium composition are evaluated. The results showed a marked effect of crude glycerol concentration on the product yield. The competitive nature of butanol and 1,3-PDO pathways has been evident, and a shift to the butanol pathway once using higher substrate concentrations (up to 35 g l −1 ) was clearly observed. Butyrate supplementation to the culture medium resulted in a 45% higher butanol titre, a lower production of 1,3-PDO and it decreased the fermentation time. Acetate supplementation also increased the butanol titre but the fermentation was longer. Even though glycerol consumption could not be increased over 32 g l −1 , when the concentrations of NH 4 Cl and FeCl 2 were simultaneously increased, the results obtained were similar to those observed when butyrate was supplemented to the culture medium; a 35% higher butanol yield at the expense of 1,3-PDO and a shorter fermentation. The results herein gathered suggest that there are other factors besides butanol inhibition and nutrient limitation that affect the glycerol consumption.
Starting Page	134
Ending Page	143
Page Count	10
File Format	PDF HTM / HTML
DOI	10.1016/j.biombioe.2014.10.015
Volume Number	71
Alternate Webpage(s)	http://repositorium.sdum.uminho.pt/bitstream/1822/31998/1/document_18494_1.pdf
Alternate Webpage(s)	https://doi.org/10.1016/j.biombioe.2014.10.015
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article