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Evaluación de nematodos entomopatógenos para el control del picudo de la piña en el estado Táchira Venezuela
| Content Provider | Semantic Scholar |
|---|---|
| Author | García-Caicedo, María M. Torres, Ángel Ochoa, Ángel |
| Copyright Year | 2013 |
| Abstract | espanolEl cultivo de la pina, Ananas comosus (L.) Merril, se ve afectado por la incidencia de diversos insectos plaga, entre ellos el coleoptero Metamasius dimidiatipennis(Jekel), conocido como el picudo de la pina, que ocasiona danos al perforar tallos y hojas. Para su control se evaluaron cepas nativas y foraneas de nematodos entomopatogenos (NEPs) pertenecientes a los generos Steinernema y Heterorhabditis (cepas 044, 037, 075, SCALL y HP88) en condiciones de laboratorio en las instalaciones del Instituto Nacional de Investigaciones Agricolas (INIA-Tachira). Se utilizaron larvas del insecto en sus ultimos dos estadios, colectados en plantaciones de pina, en el municipio Libertad, estado Tachira. Se determinaron tiempos letales (TL50 y TL95), dosis letales (DL50 y DL95) y un modelo de regresion tiempo-mortalidad para cada cepa de NEPs en el estadio juvenil infectivo (JI). Se evaluaron diferentes concentraciones de NEPs: 0, 100, 300, 500, 700 y 1.000 JI/ml, con cuatro repeticiones de cinco larvas de picudo, cada una en placas de Petri y se registro el numero de individuos muertos cada 24 h durante 6 dias. Las larvas muertas por nematodos se reconocian por presentar una consistencia suave y olor no fetido. Entre las cepas nativas, 075 obtuvo mejor resultado con un DL50 y TL50 de 500 JI/ml y 2,05 dias, respectivamente, seguida de 044 y 037, todas pertenecientes al genero Steinernema. EnglishThe pineapple, Ananas comosus L., known as the queen of the fruits, is used for fresh consumption, alcoholic drinks, pharmaceutical use, and fiber production. However, its production is affected by the incidence of various insect pests including Metamasius dimidiatipennis, known as the pineapple weevil. This insect infests the crop by perforating stems and leaves. For controlling this insect, native and foreign strains of entomopathogenic nematodes of the genus Steinernema and Heterorhabditis (Strains 044, 037, 075, SCALL, and HP88) were used under laboratory conditions at the INIA Tachira. Larvae of the insect in its last two stadia were collected from infested plants at Libertad County in Tachira. Lethal times (TL50 and TL95), lethal doses (DL50 and DL95) and a regression model of time Vs mortality were determined for each strain of entomopathogenic nematodes (EPNs) in its infective juvenile stage (IJ) at different concentrations: 0, 100, 300, 500, 700 and 1000 IJ/ml. Four replicates with 5 larvae each were placed in petri dishes along with the suspension of IJ added at each dose. The number of individuals killed was counted every 24 hours for 6 days. The nematode infested dead larvae were recognized for its soft consistency and no stinking smell. The native strain 075 gave the best performance with a DL50 and TL50 of 500 IJ/ml and 2,05 days, respectively, followed by strains 044 and 037, all in the genus Steinernema |
| Starting Page | 5 |
| Ending Page | 14 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Volume Number | 63 |
| Alternate Webpage(s) | http://www.scielo.org.ve/pdf/at/v63n1-2/art01.pdf |
| Alternate Webpage(s) | http://sian.inia.gob.ve/revistas_ci/Agronomia%20Tropical/at63_12/pdf/at63_12_garcia.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
