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Avaliação da atividade antioxidante e alelopática do extrato etanólico e frações das cascas do caule de Zanthoxylum rhoifolium Lam., Rutaceae
| Content Provider | Semantic Scholar |
|---|---|
| Author | Turnes, Joelle De Melo Bonetti, Aline F. Krause, Mariana Saragioto Canteli, Vanessa Cristina Dias Paula, Cristiane Da Silva Duarte, Márcia Do Rocio Zanin, Sandra Maria Warumby Dias, Josiane De Fátima Gaspari Miguel, Marilis Dallarmi Miguel, Obdulio Gomes |
| Copyright Year | 2015 |
| Abstract | Este trabalho teve como objetivo o estudo das atividades antioxidante e alelopatica das cascas do caule de Zanthoxylum rhoifolium Lam., Rutaceae, de modo a conduzir a descoberta de substâncias biologicamente ativas. O material vegetal foi submetido a extracao etanolica e este extrato foi fracionado obtendo as fracoes (hexano, cloroformio, acetato de etila e hidroalcoolica). Para a avaliacao da atividade antioxidante, empregaram-se os metodos de reducao do complexo fosfomolibdenio, de reducao do radical DPPH e das substâncias reativas ao acido tiobarbiturico (TBARS). Quanto a alelopatia, as amostras foram testadas em quatro concentracoes sobre a germinacao e o desenvolvimento de radicula e hipocotilo das sementes de Lactuca sativa. As amostras evidenciaram atividade antioxidante significativa frente ao metodo de reducao do complexo fosfomolibdenio quando comparada a rutina, e do TBARS quando comparado ao BHT, assim como a atividade alelopatica, uma vez que estimularam tanto a germinacao como o crescimento das sementes. A fracao cloroformica e acetato de etila demonstraram melhor potencial antioxidante com 204,17% e 127,11% em relacao a rutina no metodo de formacao do complexo fosfomolibdenio, e o extrato bruto e a fracao hexano com 64,2% e 60,9%, em relacao ao BHT, no metodo TBARS. No ensaio alelopatico, destaca-se a fracao cloroformica, pois foi a unica amostra que estimulou o crescimento do hipocotilo e radicula na maioria das concentracoes, variando de 41 a 144%, e a fracao acetato de etila que apresentou a maior porcentagem de estimulo nesse bioensaio, demonstrando estimulo de 274% do crescimento do hipocotilo. Este e o primeiro trabalho que demonstra a atividade antioxidante e alelopatica de Z. rhoifolium. Palavras-chave: Zanthoxylum rhoifolium Lam. DPPH. Fosfomolibdenio. Alelopatia. TBARS. ABSTRACT Assessment of antioxidant and allelopathic activities of ethanolic extract and solvent fractions from stem bark of Zanthoxylum rhoifolium Lam. (Rutaceae) The purpose of this study was to test for antioxidant and allelopathic activities in stem bark of Zanthoxylum rhoifolium Lam., Rutaceae, with the eventual aim of discovering biologically active substances. The plant material was subjected to ethanolic extraction and this extract was partitioned, yielding hexane, chloroform, ethyl acetate and hydroalcoholic fractions. Antioxidant activity was estimated by the reduction of phosphomolybdenum complex, of DPPH• and of thiobarbituric acid-reactive substances (TBARS). To detect allelopathy, the samples were tested, at four concentrations, on the germination and development of the radicle and hypocotyl of L. sativa seeds. The samples showed significant antioxidant activity against the reduction of the phosphomolybdenum complex, as compared to rutin, and reduction of TBARS, as compared to BHT, as well as allelopathic activity, since they stimulated growth and seed germination. The chloroform and ethyl acetate fractions showed the best antioxidant potentials, with 204.17% and 127.11% compared to rutin, in the reduction of phosphomolybdenum complex, as did the crude ethanolic extract and hexane fraction, with 64.2% and 60.9% compared to BHT, in the TBARS method. In the allelopathic assay, the chloroform fraction stood out as the only sample that stimulated the growth of both the radicle and hypocotyl at most concentrations, ranging from 41 to 144%, while the ethyl acetate fraction achieved the greatest stimulus in this bioassay, increasing the growth of the hypocotyl by 274%. This is the first study that demonstrates the antioxidant and allelopathic activities of the species Z. rhoifolium. Keywords: Zanthoxylum rhoifolium Lam. DPPH. Phosphomolybdenum. Allelopathy. TBARS. |
| Starting Page | 467 |
| Ending Page | 467 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Volume Number | 35 |
| Alternate Webpage(s) | http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewFile/2951/1610 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
