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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chomczyński, Piotr Sacchi, Nicoletta |
| Copyright Year | 1987 |
| Abstract | A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples. |
| Starting Page | 69 |
| Ending Page | 78 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.openrev.org/sites/default/files/papers/9624/custom.pdf?serve=download |
| PubMed reference number | 2440339v1 |
| Volume Number | 162 |
| Issue Number | 1 |
| Journal | Analytical biochemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Chloroform Guanidine Phenols Quantity RNA Isolation Thioctic Acid Thiocyanates phenol 14 MG/ML Mucosal Spray |
| Content Type | Text |
| Resource Type | Article |
