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An alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Coura, Renata Dos Santos Prolla, João Carlos Meurer, Luíse Ashton-Prolla, Patrícia |
| Copyright Year | 2005 |
| Abstract | BACKGROUND DNA extraction from paraffin wax embedded tissue requires special protocols, and most described methods report an amplification success rate of 60-80%. AIMS To propose a simple and inexpensive protocol consisting of xylene/ethanol dewaxing, followed by a kit based extraction. METHOD Xylene/ethanol dewaxing was followed by a long rehydration step and a kit based DNA extraction step. RESULTS This method produced a 100% amplification success rate for fragments of 121 to 227 bp for tamponated formalin fixed paraffin wax embedded tissue. CONCLUSION This cost effective and non-laborious protocol can successfully extract DNA from tamponated formalin fixed paraffin wax embedded tissue and should facilitate the molecular analysis of a large number of archival specimens in retrospective studies. |
| File Format | PDF HTM / HTML |
| DOI | 10.1136/jcp.2004.021352 |
| PubMed reference number | 16049299 |
| Journal | Medline |
| Volume Number | 58 |
| Issue Number | 8 |
| Alternate Webpage(s) | http://jcp.bmj.com/content/jclinpath/58/8/894.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1136/jcp.2004.021352 |
| Journal | Journal of clinical pathology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |