Burkholderiales participating in pentachlorophenol biodegradation in iron-reducing paddy soil as identified by stable isotope probing.

Content Provider	Semantic Scholar
Author	Tong, Hui Li, Fangbai Chen, Manjia Lv, Yahui
Copyright Year	2015
Abstract	As the most prevalent preservative worldwide for many years, pentachlorophenol (PCP) has attracted much interest in the study of biodegradation in soil and aquatic ecosystems. However, the key microorganisms involved in anaerobic degradation are less well understood. Hence, we used DNA-based stable isotope probing (SIP) to identify the PCP-degrading microorganisms in iron-rich paddy soil under anaerobic conditions. (12)C- and (13)C-labeled PCP were almost completely degraded in 30 days under iron-reducing conditions. The results of terminal restriction fragment length polymorphism (T-RFLP) of 16S rRNA genes showed that 197 and 217 bp (HaeIII digests) restriction fragments (T-RFs) were enriched in heavy DNA fractions of (13)C-labeled samples, and the information from 16S rRNA gene clone libraries suggested that the microorganisms corresponding to these T-RF fragments, which increased in relative abundance during incubation, belonged to the order of Burkholderiales, in which 197 and 217 bp were classified as unclassified Burkholderiales and the genus Achromobacter, respectively. The results of the present study indicated that Burkholderiales-affiliated microorganisms were responsible for PCP degradation in anaerobic paddy soil and shed new light on in situ bioremediation in anaerobic PCP contaminated soil.
File Format	PDF HTM / HTML
DOI	10.1039/c4em00530a
PubMed reference number	26051859
Journal	Medline
Volume Number	17
Issue Number	7
Alternate Webpage(s)	http://www.rsc.org/suppdata/em/c4/c4em00530a/c4em00530a1.pdf
Alternate Webpage(s)	https://doi.org/10.1039/c4em00530a
Journal	Environmental science. Processes & impacts
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article