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Multiplex Ligation-dependent Probe Amplification (MLPA)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Berbegall, Ana P. Villamón, Eva Navarro, Samuel Noguera, Rosa |
| Copyright Year | 2011 |
| Abstract | The Multiplex Ligation-dependent Probe Amplification (MLPA) is a PCR-based method. The procedure relies on sequence-specific probe hybridization of genomic DNA, followed by multiplex-PCR amplification of the hybridized probe and a semiquantitative analysis of the resulting PCR products. MLPA allows the analysis of around 40 loci in the same reaction, and is a sensitive and relatively fast technique. Only a small amount of DNA is required and results are available within 2 days.The critical factors when performing MLPA analyses from formalin-fixed paraffin-embedded (FFPE) tissues are DNA integrity and purity; for this reason, a suitable DNA extraction method must be chosen.The MLPA protocol described in this chapter provides a methodology for analyzing the genetic copy number changes from FFPE tissues using MLPA P-products (MRC-Holland). The steps include DNA extraction from tissue sections, DNA sample denaturation, and MLPA data analysis. |
| Starting Page | 215 |
| Ending Page | 224 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/978-3-642-17890-0_33 |
| Alternate Webpage(s) | http://www.mlpa.com/WebForms/WebFormDBData.aspx?Tag=_zjCZBtdOUyAt3KF3EwRZhCATZhnORpCBrCEQLdKlKkAOBU5tP_FbWg.. |
| Alternate Webpage(s) | https://doi.org/10.1007/978-3-642-17890-0_33 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
