NDLI: Isolation and Morphological Characterization of Ustilaginoidea Virens, Causing False Smut of Rice

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Isolation and Morphological Characterization of Ustilaginoidea Virens, Causing False Smut of Rice

Content Provider	Semantic Scholar
Author	Baite, Mathew S.
Copyright Year	2012
Abstract	False smut of rice, caused by Ustilaginoidea virens (Cooke) Takahashi (Teleomorph: Villosiclava virens Tanaka), is an important emerging disease. The conditions for the successful isolation of the pathogen in axenic cultures have been standardized. Surface sterilization with 1% sodium hypochlorite solution was found to give better result for isolation of the pathogen. The optimized conditions for the growth of the U. virens were found to be as; potato sucrose agar medium, ph 6.0 and incubation at 27oC temperature in dark. In these conditions, the fastest growth rate of the fungus was achieved which varied from 25-40 mm in different isolates. The colony appeared flat or raised with creamy white fluffy mycelium which was compact and leathery. The isolated fungus was identified according to cultural descriptions given by Sharma and Joshi (1975) and Verma and Singh (1988) and was further confirmed through ITS sequencing which showed 98-99 per cent identity with Ustilaginoidea virens in NCBI-BLAST analysis. Conidia sizes were different in different isolates (4.20–6.54 μm). The largest conidia was found in the isolate, UV5 (6.54 μm) while the smallest was recorded for the isolate, UV10 (4.20 μm). The Scanning Electron Microscopy (SEM) studies revealed the conidia as spherical, globose to irregularly rounded and ornamented with prominent spines. The spines were pointed at the apex or irregularly curved, and were 359.9-994.5 nm long. Lastly, the genetic diversity analysis with Random Amplified Polymorphic DNA (RAPD) marker with 9 random primers showed a dendrogram with two main clusters; Cluster I consists of 5 isolates: UV1, UV5, UV8, UV6 and UV7, while Cluster II consists of 3 isolates: UV2, UV3 and UV4. RAPD analysis revealed relatively high genetic diversity among Ustilaginoidea virens isolates collected from different places of India. Thus, all the isolates showed significant morphological, cultural and molecular variation.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://krishikosh.egranth.ac.in/bitstream/1/75758/1/MATHEW%20THESIS.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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