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In vivo analysis of Saccharomyces cerevisiae COX2 mRNA 5'-untranslated leader functions in mitochondrial translation initiation and translational activation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dunstan, H. Mainwaring Green-Willms, N. S. Fox, Thomas D. |
| Copyright Year | 1997 |
| Abstract | We have used mutational and revertant analysis to study the elements of the 54-nucleotide COX2 5'-untranslated leader involved in translation initiation in yeast mitochondria and in activation by the COX2 translational activator. Pet111p. We generated a collection of mutants with substitutions spanning the entire COX2 5'-UTL by in vitro mutagenesis followed by mitochondrial transformation and gene replacement. The phenotypes of these mutants delimit a 31-nucleotide segment, from -16 to -46, that contains several short sequence elements necessary for COX2 5'-UTL function in translation. The sequences from -16 to -47 were shown to be partially sufficient to promote translation in a foreign context. Analysis of revertants of both the series of linker-scanning alleles and two short deletion/ insertion alleles has refined the positions of several possible functional elements of the COX2 5'-untranslated leader, including a putative RNA stem-loop structure that functionally interacts with Pet111p and an octanucleotide sequence present in all S. cerevisiae mitochondrial mRNA 5'-UTLs that is a potential rRNA binding site. |
| Starting Page | 87 |
| Ending Page | 100 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 9286670 |
| Journal | Medline |
| Volume Number | 147 |
| Issue Number | 1 |
| Alternate Webpage(s) | http://www.genetics.org/content/genetics/147/1/87.full.pdf |
| Journal | Genetics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
