Clonotypic heterogeneity of Lewis rat T cells specific for the encephalitogenic 68-86 region of myelin basic protein.

Content Provider	Semantic Scholar
Author	Mannie, Mark D. Paterson, Philip Y. U'prichard, David C. Thomas, David William
Copyright Year	1989
Abstract	Experimental autoimmune encephalomyelitis was induced in a Lewis rat by sensitization with synthetic peptide GP68-86, representing the 68-86 sequence of guinea pig myelin basic protein (GPMBP). To delineate T cell determinants of GP68-86, lymph node cells from this rat were activated in culture with GP68-86 and were fused with cells of the mouse thymoma BW5147. The resultant hybrids were cloned by limiting dilution and screened for GP68-86-evoked secretion of IL2 in the presence of rat splenocytes. Twelve T cell hybrids derived in this manner were tested for reactivity to different heterologous species of MBP as well as to substituted or truncated analogs of GP68-86. The hybrids generally exhibited potent reactivity to GPMBP but differed markedly in their reactivity to autologous rat MBP (RMBP). A few exceptional hybrids exhibited crossreactivity with peptides in which native serine75 or serine80 residues of GPMBP were substituted with either alanine75 (A75) or proline80 (P80) residues. These cross-reactive hybrids also possessed high levels of anti-RMBP reactivity. The remaining hybrids were unresponsive to the A75 and P80 substituted peptides and, with one exception, had relatively low levels of anti-RMBP reactivity. Unique reactivity patterns were also revealed by hybrid responses to peptides having modified C-terminal 84-86 residues. In summary, the contrasting fine specificities of different hybrids indicated that several distinct clones of T cells mediate the immune response of Lewis rats against the 68-86 region of GPMBP. Furthermore, heterogeneity in the hybrid response to "self" RMBP may reflect substantial differences in encephalitogenic potency of the T cell clones from which these hybrids were derived.
File Format	PDF HTM / HTML
DOI	10.1016/0008-8749(89)90099-3
PubMed reference number	2475260
Journal	Medline
Volume Number	122
Issue Number	2
Alternate Webpage(s)	https://deepblue.lib.umich.edu/bitstream/handle/2027.42/27792/0000190.pdf?isAllowed=y&sequence=1
Alternate Webpage(s)	https://doi.org/10.1016/0008-8749%2889%2990099-3
Journal	Cellular immunology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article