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Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Grayson, Mitchell H. Hotchkiss, Richard S. Karl, Irene E. Holtzman, Michael J. Chaplin, David D. |
| Copyright Year | 2003 |
| Abstract | Lymphocyte rolling velocity is determined largely by interactions between leukocyte alpha(4)-integrin (CD49d) and L-selectin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mesenteric postcapillary venules and Peyer's patch high endothelial venules (HEVs). The role of these interactions in other tissue sites of lymphocyte emigration is not known. With the use of real-time intravital confocal microscopy, we found that rolling velocities of T lymphocytes in the murine mesenteric lymph node (MLN) HEV also depend on L-selectin and CD49d. However, in the murine spleen, rolling velocities of T lymphocytes are not influenced by the loss of L-selectin and CD49d. With the use of FITC-dextran and TIE2-GFP mice, we further defined the microvascular compartments of the spleen and showed that adherence of T cells is localized to regions in the white pulp that are not lined by endothelial cells and have shear rates similar to bone marrow sinusoids. These results establish that T cell trafficking to the spleen differs from trafficking to other secondary lymphoid organs and suggest that the mechanical properties of the blood-filtering role of the spleen are important in T cell accumulation in the organ. |
| Starting Page | 1442 |
| Ending Page | 1442 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajpheart.physiology.org/content/ajpheart/284/6/h2213.full.pdf |
| PubMed reference number | 12586641v1 |
| Volume Number | 284 |
| Issue Number | 6 |
| Journal | American journal of physiology. Heart and circulatory physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Anatomic Node Bone Marrow Cell Adhesion Cellular Phone Dental Pulp Dextrans E-Selectin Fluorescein-5-isothiocyanate Integrin alpha4 Iron-Dextran Complex Leukemia, B-Cell Leukocytes Lymph node of mesentery Lymphocyte Count measurement MADCAM1 gene MADCAM1 protein, human Malpighian corpuscles Mesenteric Lymphadenitis Mucous Membrane Organ Selectins Spleen Tissue Structure of aggregated lymphoid follicle of small intestine Structure of venule lymph nodes |
| Content Type | Text |
| Resource Type | Article |
