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Reconstruction of Highly Proliferative Auto-Tissue-Engineered Lamellar Cornea Enhanced by Embryonic Stem Cell.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhou, Qiang Liu, Zhao Wu, Zheng Wang, Xiaoran Wang, Bowen Li, Chaoyang Liu, Ying Li, Liangliang Wan, Pengxia Huang, Zheqian Wang, Zhichong |
| Copyright Year | 2015 |
| Abstract | To increase the epithelial proliferation of an auto-tissue-engineered lamellar cornea, 3.0 × 10(6) corneal epithelial cells (CECs) were combined with 3 × 10(5) mouse embryonic stem cells (ESCs) pretransfected with the HSV-tk gene (CECs + ESCs-TK group), and 3.3×10(6) corneal epithelial cells (CECs group) were seeded between the acellular porcine corneal stroma and the amniotic membrane using the centrifugal cell seeding method. After 4 days of perfusion culture (treatment with ganciclovir starting on day 2), a thicker corneal epithelium (four to five layers) formed in the CECs + ESCs-TK group compared with that observed in the CECs group (two to three layers). More stem/progenitor cell (K3 -, p63+, ABCG2+, and integrin-β1+) and proliferation phenotypes (Ki67+) were measured in the CECs + ESCs-TK group compared with the CECs group using immunofluorescence staining, real-time quantitative reverse transcription polymerase chain reaction, and flow cytometry. Consistent with these findings, the colony-forming efficiency and cellular doubling time were significantly different between the CECs + ESCs-TK group (16.18% ± 3.98%, 28.45 ± 2.03 h) and CECs group (11.96% ± 2.60%, 36.3 ± 1.15 h). In a rabbit lamellar transplantation model, the CECs + ESCs-TK group had better epithelial barrier functions and wound healing abilities compared with the CECs group. Furthermore, ESCs-TK could be completely and safely removed by ganciclovir. Thus, the ESCS-TK coculture system could serve as a potential strategy for corneal tissue engineering. |
| File Format | PDF HTM / HTML |
| DOI | 10.1089/ten.TEC.2014.0481 |
| PubMed reference number | 25425425 |
| Journal | Medline |
| Volume Number | 21 |
| Issue Number | 7 |
| Alternate Webpage(s) | https://www.uni-regensburg.de/Anatomie/Minuth/PDF/Proceedings/2015_Zhou.pdf |
| Alternate Webpage(s) | https://doi.org/10.1089/ten.TEC.2014.0481 |
| Journal | Tissue engineering. Part C, Methods |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
