Efficient generation of B lymphocytes by recognition of self-antigens.

Content Provider	Semantic Scholar
Author	Eschbach, Cathrin Bach, Martina P. Fidler, Ingrid Pelanda, Roberta Köhler, Fabian Rajewsky, Klaus Jumaa, Hassan
Copyright Year	2011
Abstract	Antibody diversity is generated by a random gene recombination process with the inherent risk of the production of autoreactive specificities. The current view suggests that B cells expressing such specificities are negatively selected at an early developmental stage. Using the knock-in model system of the 3-83 autoreactive B-cell antigen receptor (BCR) in combination with precursor-BCR (pre-BCR) deficiency, we show here that the 3-83 BCR mediates efficient generation of B cells in the presence, but not the absence, of a strongly recognized auto-antigen. Experiments with mixed bone marrow chimeras showed that combining the 3-83 BCR with the corresponding auto-antigen resulted in efficient reconstitution of B-cell development in immune-deficient mice. These results suggest that B cells are positively selected by recognition of self-antigens during developmental stages that precede receptor editing. Moreover, the data indicate that the pre-BCR functions as a specialized autoreactive BCR to initiate positive selection at a stage where the cells express immunoglobulin heavy but not light chains.
File Format	PDF HTM / HTML
DOI	10.1002/eji.201041344
PubMed reference number	21604259
Journal	Medline
Volume Number	41
Issue Number	8
Alternate Webpage(s)	https://www.sfb746.uni-freiburg.de/Publications/Jumaa/efficient-generation-of-b-lymphocytes-by-recognition-of-self-antigens.pdf
Alternate Webpage(s)	https://doi.org/10.1002/eji.201041344
Journal	European journal of immunology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article