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Modulation of corneal epithelial cell functions by the neutrophil-derived inflammatory mediator CAP37.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Pereira, H. Anne Ruan, Xin Gonzalez, Melva L. Tsyshevskaya-Hoover, Irina Chodosh, James |
| Copyright Year | 2004 |
| Abstract | PURPOSE To investigate the effect of CAP37, an inflammatory mediator in neutrophils, on three important events in corneal wound healing: proliferation, migration, and adhesion. METHODS Immortalized human corneal epithelial cells (HCEC) were treated with CAP37, and its effects on migration and proliferation were measured using the modified Boyden chemotaxis chamber and the proliferation assays (CyQUANT; Molecular Probes, Eugene, OR), respectively. Effects on adhesion were determined by measuring upregulation of adhesion molecules belonging to the selectin, integrin, and immunoglobulin superfamily using RT-PCR and flow cytometry. RESULTS CAP37 promoted proliferation of HCEC in a time- and dose-dependent fashion. CAP37 was maximally chemotactic for HCEC over a range of 1.3 x 10(-8) to 5.2 x 10(-8) M. CAP37 upregulated intercellular adhesion molecule (ICAM)-1, platelet endothelial cell adhesion molecule (PECAM)-1, and integrin molecules alpha3 (CD49c) and beta1 (CD29). Data on migration and ICAM-1 and PECAM-1 upregulation were corroborated using primary human corneal epithelial cells. CONCLUSIONS CAP37 modulated corneal epithelial cell proliferation and migration and upregulated adhesion molecules involved in leukocyte-epithelial and epithelial-extracellular matrix interactions. |
| Starting Page | 241 |
| Ending Page | 245 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.iovs.org/content/45/12/4284.full.pdf |
| PubMed reference number | 15557434v1 |
| Volume Number | 45 |
| Issue Number | 12 |
| Journal | Investigative ophthalmology & visual science |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Blood Platelets Cell Adhesion Molecules Cell physiology Endothelial Cells Epithelial Cell Proliferation Epithelial Cells Extracellular Matrix Flow Cytometry Integrin alpha3 Leukocytes Mediator brand of benfluorex hydrochloride Platelet Endothelial Cell Adhesion Molecule, human Reverse Transcriptase Polymerase Chain Reaction Selectins Up-Regulation (Physiology) Wound Infection cationic antimicrobial protein CAP 37 negative regulation of leukocyte cell-cell adhesion neutrophil |
| Content Type | Text |
| Resource Type | Article |
