Loading...
Please wait, while we are loading the content...
Similar Documents
A rapid and simple method for extracting yeast mitochondrial DNA
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gargouri, Ali |
| Copyright Year | 2004 |
| Abstract | SummaryA rapid method for the extraction of yeast mitochondrial DNA (mtDNA) is described. In comparison with previous methods, it simplifies several steps, does not require either the isolation of mitochondria or phenol treatment and is less time consuming. This protocol gives a high yield of pure mtDNA (50–120 μg from a 100-ml culture), which can be directly used in various molecular applications: restriction enzyme digestion, electrophoresis, blotting, labeling, cloning and sequencing. |
| Starting Page | 235 |
| Ending Page | 237 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/BF00435511 |
| Volume Number | 15 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/BF00435511 |
| Alternate Webpage(s) | https://doi.org/10.1007/BF00435511 |
| Journal | Current Genetics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
