The avian retroviral IN protein is both necessary and sufficient for integrative recombination in vitro

Content Provider	Semantic Scholar
Author	Katz, Richard A. Merkel, George Kulkosky, Joseph Leis, Jonathan P. Skalka, Anna Marie
Copyright Year	1990
Abstract	The integration of viral DNA into the host cell chromosome is an essential feature of the retroviral life cycle. The integration reaction requires cis-acting sequences at the ends of linear viral DNA and a trans-acting product of the pol gene, the integration protein (IN). Previously, we demonstrated that avian sarcoma-leukosis virus (ASLV) IN is able to carry out the first step in the integration process in vitro: nicking of the ends of linear viral DNA. In this paper, using two independent assays, we demonstrate that IN, alone, is sufficient to carry out the second step: cleavage and joining to the target DNA. These results demonstrate that the retroviral IN protein is an integrase.
Starting Page	87
Ending Page	95
Page Count	9
File Format	PDF HTM / HTML
DOI	10.1016/0092-8674(90)90290-U
PubMed reference number	2170022
Journal	Medline
Volume Number	63
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/009286749090290U
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/009286749090290U?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/0092-8674%2890%2990290-U
Journal	Cell
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article