NDLI: Chloroplast membranes of the green alga Acetabularia mediterranea. II. Topography of the chloroplast membrane

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Chloroplast membranes of the green alga Acetabularia mediterranea. II. Topography of the chloroplast membrane

Content Provider	Semantic Scholar
Author	Apel, Kenn Miller, Kenneth Richard Bogorad, L. Miller, Gordon J.
Copyright Year	1976
Abstract	The localization of the chlorophyll-protein complexes inside the thylakoid membrane of Acetabularia mediterranea was determined by fractionating the chloroplast membrane with EDTA and Triton X-100, by using pronase treatment, and by labeling the surface-exposed proteins with 125I. The effects of the various treatments were established by electrophoresis of the solubilized membrane fractions and electron microscopy. After EDTA and pronase treatment, the membrane structure was still intact. Only the two chlorophyll-protein complexes of 67,000 and 152,000 daltons and an additional polypeptides were found in the membrane before the EDTA and pronase treatment. The 125,000 dalton complex seems to be buried inside the lipid layer. The 23,000 dalton subunit of the 67,000 dalton complex is largely exposed to the surface of the EDTA-insoluble membrane and only the chlorophyll-binding subunit of 21,500 daltons is buried inside the lipid layer.
Starting Page	876
Ending Page	893
Page Count	18
File Format	PDF HTM / HTML
DOI	10.1083/jcb.71.3.876
PubMed reference number	825523
Journal	Medline
Volume Number	71
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/71/3/876.full.pdf
Alternate Webpage(s)	https://doi.org/10.1083/jcb.71.3.876
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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