Macromolecular properties and end-group analysis of heparin isolated from bovine liver capsule.

Content Provider	Semantic Scholar
Author	Jansson, Leif Niclas Ogren, S. Lindahl, Ulf
Copyright Year	1975
Abstract	Glycosaminoglycans were extracted from bovine liver capsule with 4 M-guanidinium chloride, resulting in solubilization of approx. 90% of the total uronic acid-containing polysaccharide of the tissue. The extracted polysaccharide was purified and fractionated by anion-exchange chromatography on DEAE-cellulose, density-gradient ultracentrifugation in CsCl and finally gel chromatography on Sepharose 4B. By using these procedures, the two major polysaccharide components, dermatan sulphate and heparin, which constituted 55 and 30% respectively of the total glycosaminoglycan content of the tissue, were separated from each other. Analysis of the macromolecular properties of the two polysaccharides showed that heparin existed exclusively as single polysaccharide chains, whereas dermatan sulphate occurred largely as a proteoglycan (protein content, 74% dry wt.). The purified heparin preparation was subjected to sedimentation-equilibrium ultracentrifugation, indicating a molecular weight of 8800. Analysis for neutral sugars (by g.l.c.) showed 0.1 residue of xylose and 0.2 residue of galactose/polysaccharide chain; serine amounted to 0.3 residue/polysaccharide chain. Reduction of the heparin with NaB3H4 resulted in incorporation of 3H, approximately corresponding to one reducible group/polysaccharide chain. The 3H-labelled sugar residue was liberated by a combination of acid hydrolysis and deaminative cleavage of the polysaccharide with HNO2; it was subsequently identified as an aldonic acid by paper electrophoresis. Most of the heparin chains thus contained a uronic acid residue in reducing position. It is suggested that heparin isolated from bovine liver capsule is a degradation product released from larger molecules by an endo-glycuronidase.
Starting Page	53
Ending Page	62
Page Count	10
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.biochemj.org/content/ppbiochemj/145/1/53.full.pdf
PubMed reference number	127581v1
Volume Number	145
Issue Number	1
Journal	The Biochemical journal
Language	English
Access Restriction	Open
Subject Keyword	2-diethylaminoethanol Anions Cattle Cellulose Chloride Ion Contain (action) DEAE-Cellulose Dermatan Sulfate Electrophoresis Electrophoresis, Paper Endometriosis Extraction Galactose Gel Chromatography Gradient Guanidine Hydrochloride Large Molecular Weight Polysaccharides Proteoglycan Sedimentation procedure Sugar Alcohol Dehydrogenases Sugars Sulfate measurement Thioctic Acid Ultracentrifugation Xylose heparin
Content Type	Text
Resource Type	Article