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Detection of a single enzyme molecule based on a solid-state nanopore sensor.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tan, Shengwei Gu, Dejian Liu, Hang Liu, Quanjun |
| Copyright Year | 2016 |
| Abstract | The nanopore sensor as a high-throughput and low-cost technology can detect a single molecule in a solution. In the present study, relatively large silicon nitride (Si3N4) nanopores with diameters of ∼28 and ∼88 nm were fabricated successfully using a focused Ga ion beam. We have used solid-state nanopores with various sizes to detect the single horseradish peroxidase (HRP) molecule and for the first time analyzed single HRP molecular translocation events. In addition, a real-time monitored single enzyme molecular biochemical reaction and a translocation of the product of enzyme catalysis substrates were investigated by using a Si3N4 nanopore. Our nanopore system showed a high sensitivity in detecting single enzyme molecules and a real-time monitored single enzyme molecular biochemical reaction. This method could also be significant for studying gene expression or enzyme dynamics at the single-molecule level. |
| Starting Page | 155502 |
| Ending Page | 155502 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1088/0957-4484/27/15/155502 |
| PubMed reference number | 26937593 |
| Journal | Medline |
| Volume Number | 27 |
| Issue Number | 15 |
| Alternate Webpage(s) | http://iopscience.iop.org/article/10.1088/0957-4484/27/15/155502/pdf |
| Alternate Webpage(s) | https://doi.org/10.1088/0957-4484%2F27%2F15%2F155502 |
| Journal | Nanotechnology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
