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Transformation of Pyrococcus furiosus with Thermostable Green Fluorescent Protein
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hollar, Emma R. |
| Copyright Year | 2018 |
| Abstract | Archaea are the most recently discovered domain of life, are extremely diverse and can be manipulated for research and commercial use. Pyrococcus furiosus is a hyperthermophilic archaeon used as a model organism for studying archaea, especially extremophiles. Green fluorescent proteins are a commonly used marker of transcription but can only be expressed in a narrow temperature zone and denature at the extreme optimal growth temperature of P. furiosus. To mediate this inability to use green fluorescent protein in hyperthermophiles, a shuttle vector for P. furiosus and Escherichia coli was designed and genes for thermostable green fluorescent proteins were incorporated. This plasmid can be used to upregulate expression of this highly thermostable green fluorescent protein and demonstrate successful transformation. Though the plasmid was not transformed into P. furiosus, the construction of the plasmid and transformation of E. coli were important steps that gave insight into methods of making this transformation and expression possible. Introduction Archaea are the most recently discovered and least understood domain of life. Studying their mechanisms for survival and proliferation can tell us a lot about not only our own cells but also how life developed and how to engineer biological systems to exist and flourish in any setting. First described as a distinct domain of life in the 1970s through DNA sequencing, archaea are some of the most diverse and resilient organisms on the planet (Farkas, Picking & Santangelo 2013). They thrive in conditions with extreme temperatures and salt concentrations, environments previously considered to be uninhabitable. They have also been found in the |
| File Format | PDF HTM / HTML |
| DOI | 10.15760/honors.620 |
| Alternate Webpage(s) | https://pdxscholar.library.pdx.edu/cgi/viewcontent.cgi?article=1695&context=honorstheses |
| Alternate Webpage(s) | https://doi.org/10.15760/honors.620 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
