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A Potential Mechanism for the Anti-Apoptotic Property of Koumine Involving Mitochondrial Pathway in LPS-Mediated RAW 264.7 Macrophages
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yuan, Zhi-Hang Liang, Zeng-Enni Wu, Jing Yi, Jin-E Chen, Xiaojun Sun, Zhuojian |
| Copyright Year | 2016 |
| Abstract | Koumine is a kind of alkaloid extracted from Gelsemium elegans (G. elegans). Benth, which has shown promise as an anti-tumor, anxiolytic, and analgesic agent. In our present study, the effect of koumine on lipopolysaccharide (LPS)-mediated RAW 264.7 cell apoptosis was evaluated. MTT assays showed that koumine obviously increased cell viability in LPS-mediated RAW 264.7 macrophages. Preincubation with koumine ameliorated LPS-medicated apoptosis by decreasing reactive oxygen species (ROS) production, which resulted in a significant decrease in the levels of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). In addition, koumine-pretreated RAW 264.7 macrophages exhibited reduction of LPS-induced levels of TNF-α, IL-1β, and IL-6 mRNA. Furthermore, pretreatment with koumine suppressed LPS-mediated p53 activation, loss of mitochondrial membrane potential, caspase-3 activation, decrease of Bcl-2 expression, and elevation of Bax and caspase-3 expressions, suggesting that koumine might act directly on RAW 264.7 cells to inhibit LPS-induced apoptosis. It seems as though the mechanism that koumine possesses is the anti-apoptotic effect mediated by suppressing production of ROS, activation of p53, and mitochondrial apoptotic pathways in RAW 264 cells. Koumine could potentially serve as a protective effect against LPS-induced apoptosis. |
| Starting Page | 251 |
| Ending Page | 306 |
| Page Count | 56 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.mdpi.com/1420-3049/21/10/1317/pdf |
| PubMed reference number | 27706063 |
| Volume Number | 21 |
| Journal | Molecules |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Alkaloids Analgesics Apoptosis Cell Survival Extraction Gelsemium Lipopolysaccharides Membrane Potentials Mitochondria Mitochondrial Membranes Neoplasms Nitric Oxide Synthase Reactive Oxygen Species caspase caspase-3 koumine monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
