Bioconversion of Biodiesel Derived Crude Glycerol by Immobilized Clostridium pasteurianum: Effect of Temperature

Content Provider	Semantic Scholar
Author	Khanna, Swati Goyal, Arun Moholkar, Vijayanand S.
Copyright Year	2012
Abstract	Batch fermentation of 5, 10 and 25 g/L biodiesel derived crude glycerol was carried out at 30, 37 an d 45C by Clostridium pasteurianum cells immobilized on silica. Maximum yield of 1,3-propanediol (PDO) (0.60 mol/mol), and ethanol (0.26 mol/mol) were obtained from 10 g/L crude glycerol a t 30 and 37C respectively. Maximum yield of butanol (0.28 mol/mo l substrate added) was obtained at 37 C with 25 g/L substrate. None of the three products were detected at 45 C even after 10 days of fermentation. Only traces of ethanol (0.01 mol/mol) were detected at 45C with 5 g/L substrate. The results obtained for 25 g/L subs trate utilization were fitted in first order rate equation to obtain the values of rate constant at three different temperatures for biocon version of glycerol. First order rate constants for bioconversion of gly cerol at 30, 37 and 45C were found to be 0.198, 0.294 and 0.029/day respe ctively. Activation energy (Ea) for crude glycerol bioconver sion was calculated to be 57.62 kcal/mol. Keywords—activation energy, Clostridium pasteurianum, crude glycerol, immobilization
Starting Page	289
Ending Page	292
Page Count	4
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Volume Number	6
Alternate Webpage(s)	http://waset.org/publications/4010/bioconversion-of-biodiesel-derived-crude-glycerol-by-immobilized-clostridium-pasteurianum-effect-of-temperature
Alternate Webpage(s)	http://www.idc-online.com/technical_references/pdfs/chemical_engineering/Bioconversion%20of%20Biodiesel.pdf
Alternate Webpage(s)	https://waset.org/publications/4010/bioconversion-of-biodiesel-derived-crude-glycerol-by-immobilized-clostridium-pasteurianum-effect-of-temperature
Language	English
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Content Type	Text
Resource Type	Article