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Carotenoid levels in human lymphocytes , measured by Raman microspectroscopy
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sijtsema Maas, Louis Van Der Otto, Cees |
| Copyright Year | 2004 |
| Abstract | Carotenoid levels in lymphocytes obtained from peripheral blood of healthy people have been investigated by Raman microspectroscopy. We observed that carotenoids are concentrated in so-called “Gall bodies”. The level of carotenoids in living human lymphocytes was found to be age-dependent and to decrease with age. We performed carotenoid uptake experiments using liposomes containing beta-carotene (egg phosphatidylcholine and egg phosphatidylglycerol, 10/1 molar ratio). We observed that beta-carotene is rapidly taken up in vitro, and transported to the Gall body. From these findings we conclude that Raman microspectroscopy is a sensitive method to determine carotenoid levels in single living cells. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://doc.utwente.nl/96418/1/Ramanauskaite%20(1997)%20Pure%20Appl%20Chem%2069_2131-2134.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Beta Carotene Carotenoids Concentrate Dosage Form Experiment Human body Liposomes Phosphatidyl glycerol Phosphatidylcholines Raman scattering beta Thalassemia peripheral blood |
| Content Type | Text |
| Resource Type | Article |
