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Human monoclonal anti-keyhole limpet hemocyanin antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a keyhole limpet hemocyanin-immune individual
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lane, Henry Clifford Shelhamer, James H. Mostowski, Howard S. Fauci, Anthony S. |
| Copyright Year | 1982 |
| Abstract | A human IgMk monoclonal antibody, 2F7, of predetermined specificity, has been produced by the fusion of human peripheral blood lymphocytes with the nonsecreting mouse myeloma line SP-1. The heterohybridoma has remained stable for over 8 mo, with culture supernatants containing up to 30 micrograms/ml of specific IgM. The antibody has been shown to be capable of inducing a blastogenic response in the absence of antigen in the peripheral blood lymphocytes of normal subjects immune to the antigen. The ability to choose an antigen, immunize a human subject to that antigen, and then use the peripheral blood lymphocytes from that subject to produce antigen-specific human monoclonal antibodies should be of great value in a wide variety of investigative, diagnostic, and therapeutic endeavors.U |
| Starting Page | 333 |
| Ending Page | 338 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1084/jem.155.1.333 |
| PubMed reference number | 6976416 |
| Journal | Medline |
| Volume Number | 155 |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/d0/d9/je1551333.PMC2186568.pdf |
| Alternate Webpage(s) | https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/d9/je1551333.PMC2186568.pdf |
| Alternate Webpage(s) | http://jem.rupress.org/content/jem/155/1/333.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1084/jem.155.1.333 |
| Journal | The Journal of experimental medicine |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
