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Granulicella arctica sp. nov., Granulicella mallensis sp. nov., Granulicella tundricola sp. nov. and Granulicella sapmiensis sp. nov., novel acidobacteria from tundra soil.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Männistö, Minna K. Rawat, Suman Lata Starovoytov, Valentin Häggblom, Max M. |
| Copyright Year | 2012 |
| Abstract | Four aerobic bacteria, designated MP5ACTX2(T), MP5ACTX8(T), MP5ACTX9(T) and S6CTX5A(T), were isolated from tundra soil of north-western Finland (69° 03' N 20° 50' E). Cells of all isolates were Gram-negative, non-motile rods. Phylogenetic analysis indicated that they belonged to the genus Granulicella of subdivision 1 of the phylum Acidobacteriahttp://dx.doi.org/10.1601/nm.7918. 16S rRNA gene sequence similarity between the new isolates and the type strains of Granulicella aggregans, Granulicella paludicola, Granulicella pectinivorans and Granulicella rosea ranged from 94 to 99 %. Analysis of the RNA polymerase beta subunit (rpoB) gene sequence indicated that the isolates represented novel species of the genus Granulicella (<92 % rpoB sequence similarity between the isolates and members of the genus Granulicella). This was also confirmed by low DNA-DNA relatedness (31 %) between strain S6CTX5A(T) and the type strain of G. pectinivorans, which exhibited 99.1 % 16S rRNA gene sequence similarity and 91.7 % rpoB gene sequence similarity. The isolates grew at pH 3.5-6.5 and at 4-26 °C. Sugars were the preferred growth substrates. The major cellular fatty acids were iso-C(15 : 0), C(16 : 1)ω7c and C(16 : 0) and the major isoprenoid quinone was MK-8. The DNA G+C content was 56-60 mol%. On the basis of phylogenetic analysis and chemotaxonomic and physiological data, the isolates represent four novel species of the genus Granulicella, for which the names Granulicella arctica MP5ACTX2(T) (= ATCC BAA-1858(T) = DSM 23128(T)), Granulicella mallensis MP5ACTX8(T) (= ATCC BAA-1857(T) = DSM 23137(T)), Granulicella tundricola MP5ACTX9(T) (ATCC BAA-1859(T) = DSM 23138(T)) and Granulicella sapmiensis S6CTX5A(T) (= LMG 26174(T) = DSM 23136(T)) are proposed. An emended description of the genus Granulicella is also presented. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 22058325 |
| Journal | Medline |
| Volume Number | 62 |
| Part | 9 |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/ijsem/62/9/2097_ijs031864.pdf?accname=guest&checksum=183F4325E642BDB1AE5A7A43D76F4CA7&expires=1503572372&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/ijsem/62/9/2097_ijs031864.pdf?accname=guest&checksum=31A01C7EF7F5B24943F5923989778205&expires=1557188886&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/ijsem/62/9/2097_ijs031864.pdf?accname=guest&checksum=46034268E44816C8729DD2DAB2C08789&expires=1545160891&id=id |
| Alternate Webpage(s) | https://doi.org/10.1099/ijs.0.031864-0 |
| Journal | International journal of systematic and evolutionary microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
