NDLI: Characterization, cloning and sequencing of a thermostable endo-(1,3–1,4) β-glucanase-encoding gene from an alkalophilic Bacillus brevis

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Characterization, cloning and sequencing of a thermostable endo-(1,3–1,4) β-glucanase-encoding gene from an alkalophilic Bacillus brevis

Content Provider	Semantic Scholar
Author	Louw, Maureen E. Reid, Sharon J. Watson, T. G.
Copyright Year	2004
Abstract	A Bacillus brevis gene coding for an endo-(1,3–1,4)-β-glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the β-glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1,3–1,4)-β-glucanases. The optimum temperature and pH for enzyme activity were 65–70°C and 8–10, respectively. When held at 75°C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS.
Starting Page	507
Ending Page	513
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1007/BF00242946
PubMed reference number	7763386
Journal	Medline
Volume Number	38
Alternate Webpage(s)	http://researchspace.csir.co.za/dspace/bitstream/handle/10204/2023/Louw_1993.pdf;jsessionid=AE75EC521631EDC3C5F5925E9BC7BA28?sequence=3
Alternate Webpage(s)	https://doi.org/10.1007/BF00242946
Journal	Applied Microbiology and Biotechnology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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