Mutation of ras oncogene in di-isopropanolnitrosamine-induced rat thyroid carcinogenesis

Content Provider	Semantic Scholar
Author	Kobayashi, Yoshihiko Kawaoi, Akira Katoh, Ryohei
Copyright Year	2002
Abstract	Abstract. To clarify the role of ras gene mutation in thyroid tumorigenesis, DNAs extracted from various rat thyroid lesions induced by di-isopropanolnitrosamine (DIPN) administration were analyzed using the microdissection–polymerase chain reaction–direct sequence (MD-PCR-DS) method. The MD-PCR-DS method revealed that K-ras gene mutation (G–A transition at codon 12) was frequently detected in nodular lesions (incidence of mutation 75%) and absent in diffuse hyperplastic and pre-nodular lesions. Although the incidence of mutation in nodular lesions was not correlated with the histological type (type 2A 76%; type 2B 84%; and type 3 71%) or treatment period (15 weeks 84% and 30 weeks 71%), it was correlated with the administration method (single injection 55% and serial injection 91%). In conclusion, K-ras mutation plays an important role in DIPN-induced rat thyroid tumorigenesis, possibly regarded as an early event in the tumorigenic process.
Starting Page	289
Ending Page	295
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1007/s00428-002-0608-y
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1007/s00428-002-0608-y
PubMed reference number	12242526
Alternate Webpage(s)	https://doi.org/10.1007/s00428-002-0608-y
Journal	Medline
Volume Number	441
Journal	Virchows Archiv
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article