NDLI: Cloning and characterization of a maize cDNA encoding phytoene desaturase, an enzyme of the carotenoid biosynthetic pathway

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Cloning and characterization of a maize cDNA encoding phytoene desaturase, an enzyme of the carotenoid biosynthetic pathway

Content Provider	Semantic Scholar
Author	Li, Zhou-Hui Matthews, Paul D. Burr, Benjamin Wurtzel, Eleanore T.
Copyright Year	2004
Abstract	To study regulation of the plastid-localized maize carotenoid biosynthetic pathway, a cDNA encoding phytoene desaturase (PDS) was isolated and characterized. The DNA sequence of the maize Pds cDNA was determined and compared with available dicot Pds genes. The deduced PDS protein, estimated at 64.1 kDa (unprocessed), had a dinucleotide binding domain and conserved regions characteristic of other carotene desaturases. Alignment of available PDS sequences from distantly related organisms suggests that Pds has potential as a phylogenetic tool. By use of heterologous complementation in Escherichia coli, maize PDS was shown to catalyze two desaturation steps converting phytoene to ζ-carotene. RFLP (restriction fragment length polymorphism) mapping was used to place Pds on chromosome 1S near viviparous5 (vp5), and RT-PCR (reverse-transcriptase polymerase chain reaction) analysis indicated reduced Pds transcript in vp5 mutant relative to normal endosperm. Other phytoene-accumulating mutant endosperms, vp2 and white3 (w3), showed no difference in Pds transcript accumulation as compared with normal endosperm counterparts. RT-PCR analysis of Pds transcript accumulation in developing endosperm showed Pds was constitutively expressed. Therefore, endosperm carotenogenesis is not regulated by increasing the level of Pds transcripts.
Starting Page	269
Ending Page	279
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1007/BF00020113
PubMed reference number	8616251
Journal	Medline
Volume Number	30
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1007/BF00020113
Alternate Webpage(s)	https://doi.org/10.1007/BF00020113
Journal	Plant Molecular Biology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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