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Implementación de una metodología analítica para la cuantificación de proteínas en la microalga Arthrospira platensis
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ramos, Leenin Flores Soto, Anthony Ruíz |
| Copyright Year | 2017 |
| Abstract | The Hartree-Lowry method was developed and implemented for quantification of protein in Arthorspira platensis microalgae by spectrophotometry UV-Vis. In order to achieve this goal, the protein extraction factors were optimized by Box Behnken design of response surface and the following results of the implementation parameters were obtained: the linearity showed a coefficient of determination (R2) greater than 0.995, a coefficient of variation less than 2% for repeatability, a percentage recovery within 94% to 101%, the limit of detection and limit of quantification were 2.36 μg/mL and 7.86 μg/mL respectively, and the robustness showed that some parameters such as sample weight and volume of reagent C presented high influence in the evaluated factors. |
| Starting Page | 371 |
| Ending Page | 381 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.37761/rsqp.v83i4.219 |
| Volume Number | 83 |
| Alternate Webpage(s) | http://www.scielo.org.pe/pdf/rsqp/v83n4/a02v83n4.pdf |
| Alternate Webpage(s) | https://doi.org/10.37761/rsqp.v83i4.219 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
