Loading...
Please wait, while we are loading the content...
Similar Documents
Three-dimensional image reconstruction of micrographs from a laser scanning confocal microscope of CdSe-core quantum dots in human umbilical vein endothelial cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nygren, Nelly Schiller, Carl |
| Copyright Year | 2019 |
| Abstract | Usage of Quantum Dots (QDs) for fluorescent labelling on biological samples has some favorable properties over traditional organic molecules. The intensity of the fluorescence from QDs is greater than that from some organic molecules, allowing lower excitation intensities which results in lower phototoxicity in the biological sample. QDs usually have a diameter in the the scale of a few nanometers, which is smaller than the resolution limit of confocal light microscopes. The aim of this thesis is to try to resolve more contrast and detail in micrographs of human embilical vein endothelial cells stained with QDs. The method of choice here is deconvolution with L1-norm regularization combined with Arnoldi iterations for computational optimization. The resulting deconvolutions of fluorescence micrographs have higher constrast constrained to smaller spatial regions than in the original micrographs. We conclude that the regularization implemented here could possibly be a viable alternative to commercially available deconvolution methods, but more research is needed to optimize computation speed and accuracy. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://kth.diva-portal.org/smash/get/diva2:1335351/FULLTEXT01.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
