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Human TopBP 1 Participates in Cyclin E / CDK 2 Activation and Preinitiation Complex Assembly during G 1 / S Transition *
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jeon, Yesu Lee, Kyung Yong Ko, Min Ji Lee, Yong Sun Kang, Sukhyun Hwang, Deog Su |
| Copyright Year | 2007 |
| Abstract | Human TopBP1 with eight BRCA1 C terminus domains has been mainly reported to be involved in DNA damage response pathways.Herewe show that TopBP1 is also required forG1 to S progression in a normal cell cycle. TopBP1 deficiency inhibited cells fromentering Sphase byup-regulatingp21 andp27, resulting in down-regulation of cyclin E/CDK2. Although co-depletion of p21 and p27 with TopBP1 restored the cyclin E/CDK2 kinase activity, however, cells remained arrested at the G1/S boundary, showing defective chromatin-loading of replication components. Based on these results, we suggest a dual role of TopBP1 necessary for the G1/S transition: one for activating cyclinE/CDK2kinase and the other for loading replication components onto chromatin to initiate DNA synthesis. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jbc.org/content/282/20/14882.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
