NDLI: Extracellular polymeric substance from Aphanizomenon flos-aquae induces apoptosis via the mitochondrial pathway in A431 human epidermoid carcinoma cells.

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Extracellular polymeric substance from Aphanizomenon flos-aquae induces apoptosis via the mitochondrial pathway in A431 human epidermoid carcinoma cells.

Content Provider	Semantic Scholar
Author	Xue, Xing Lv, Ying Liu, Qing Zhang, Xiaolan Zhao, Youhong Zhang, Lili Xu, Shiyuan
Copyright Year	2015
Abstract	Extracellular polymeric substance (EPS) is a substance secreted during algal growth, which has been found to have numerous health-promoting effects. In the present study, A431 human epidermoid carcinoma cells were selected as target cells and cultivated in vitro as an experimental model to investigate the anti-cancer effect of extracellular polymeric substances from Aphanizomenon flos-aquae (EPS-A) and the possible underlying mechanism. Apoptosis- and cell cycle-associated molecules as well as the mitochondrial membrane potential of the cells were quantified using flow cytometry (FCM). FCM showed that EPS-A induced cell cycle arrest, which led to a loss of mitochondrial function of the A431 cells and an increase in necrotic and late apoptotic cells. In order to evaluate the apoptosis and cell viability, acridine orange/ethidium bromide staining was used, morphological changes were observed using fluorescence microscopy and typical apoptotic characteristics were observed. Following treatment with a high dose of EPS-A, transmission electron microscopy showed nuclear fragmentation, chromosome condensation, cell shrinkage and expansion of the endoplasmic reticulum; apoptotic bodies were also observed. In conclusion, EPS-A caused cell cycle arrest, stimulated cell apoptosis via the mitochondrial pathway and exhibited important anti-cancer activity.
Starting Page	927
Ending Page	932
Page Count	6
File Format	PDF HTM / HTML
DOI	10.3892/etm.2015.2644
PubMed reference number	26622416
Journal	Medline
Volume Number	10
Issue Number	3
Alternate Webpage(s)	https://www.spandidos-publications.org/etm/10/3/927/download
Alternate Webpage(s)	https://doi.org/10.3892/etm.2015.2644
Journal	Experimental and therapeutic medicine
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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