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Oligonucleotide Primers for Amplification of the Mouse LPL Gene
| Content Provider | Semantic Scholar |
|---|---|
| Author | Xiao, Cuiying Xia, Qingjie |
| Copyright Year | 1999 |
| Abstract | be ligated to DNA fragments from any source, e.g., cDNAs obtained after subtractive hybridization or DNA fragments from S1 mapping experiments for analyzing exon-intron boundaries of genomic DNA. Due to the high ligation efficiency of dsPPL to the denatured ssDNA, the subsequent amplification is very efficient. It should be emphasized that PPL can be any palindromic structure that has three random nucleotides at its 3′ end and a ddNTP as its last nucleotide. This makes the method even more widely applicable. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.biotechniques.com/multimedia/archive/00006/98255bm06_6718a.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |