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Sequencing and characterization of trypsin modulating oostatic factor (TMOF) from the ovaries of the grey fleshfly, Neobellieria (Sarcophaga) bullata
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bylemans, Dany Borovsky, Dov Hunt, Donald F. Shabanowitz, Jeffrey Loof, Arnold De |
| Copyright Year | 1994 |
| Abstract | Injection of crude extracts of late vitellogenic ovaries into staged females of the grey fleshfly Neobellieria (Sarcophaga) bullata inhibited oocyte development and biosynthesis of trypsin-like enzymes in the gut. Trypsin synthesis in N. bullata is cyclic and is correlated with egg development, which is discontinuous. A trypsin modulating oostatic factor (Neb-TMOF) was purified from 10,000 vitellogenic ovaries and sequenced by mass spectrometry. Neb-TMOF is a hexapeptide (NH2-NPTNLH-COOH). Injection of the hormone at physiological concentrations (10(-9) M), inhibited trypsin-like synthesis by the midgut of liver-fed female flies, and caused a reduction of the vitellogenin concentration in the hemolymph and of oocyte growth. The role of Neb-TMOF in controlling egg development and the physiological similarities with Aedes-TMOF are discussed. |
| Starting Page | 61 |
| Ending Page | 72 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0167-0115(94)90192-9 |
| PubMed reference number | 8159807 |
| Journal | Medline |
| Volume Number | 50 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0167011594901929 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0167011594901929?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0167-0115%2894%2990192-9 |
| Journal | Regulatory Peptides |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
