Secreted proteins from Leptosphaeria maculans, the blackleg fungus, induce defence responses in canola leaves.

Content Provider	Semantic Scholar
Author	Wilson, Leanne M. Idnurm, Alexander Howlett, Barbara J. Edwards, J.
Copyright Year	2003
Abstract	Proteins secreted by plant pathogenic fungi are of particular interest in the host-pathogen interaction, because they are present at the interface between fungal and plant cells, and as such are candidates for roles in plant disease. Secreted proteins can be identified by analysis of the nucleotide sequences of the genes that encode them. With the advent of sequencing of random genes or complete genomes of several pathogenic fungi, a number of secreted proteins have been identified and shown to play a role in plant disease. We have characterised two such genes encoding secreted proteins (sp1 and sp2) from the blackleg fungus, Leptosphaeria maculans. The smaller protein (SP1 sized 12.3 kDa) was identified after sequencing random genes expressed by L. maculans. The second protein (SP2 sized 28.5 kDa) is secreted abundantly in liquid cultures of the fungus. Both genes are expressed during L. maculans infection of host (Brassica napus) cotyledons. Secreted protein fractions from L. maculans induce autofluorescent defence responses in B. napus leaves, which implies that these proteins play a role in disease. A L. maculans strain with a mutation in secreted protein sp1 causes similar sized lesions to those caused by the wildtype, indicating that although this protein may play a part in disease, it is not essential.
Starting Page	136
Ending Page	139
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.australianoilseeds.com/__data/assets/pdf_file/0016/4561/Secreted_proteins_from_blackleg_induce_resistance_in_canola.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article