NDLI: Resting and activated subsets of mouse multipotent hematopoietic stem cells.

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Resting and activated subsets of mouse multipotent hematopoietic stem cells.

Content Provider	Semantic Scholar
Author	Spangrude, Gerald J. Johnson, Gibbes R.
Copyright Year	1990
Abstract	The fluorescent vital dye rhodamine 123 (Rh-123), which preferentially accumulates in mitochondrial membranes, can be used as a probe to indicate mitochondrial and hence cellular activity. In this study, mouse bone marrow hematopoietic stem cells were subdivided into Rh-123lo, Rh-123med, and Rh-123hi populations. The Rh-123lo (resting) population was significantly enriched in cells with a higher proliferative potential compared to the Rh-123hi (activated) population. The resting population exhibited a 20-fold greater ability to differentiate into splenic colony-forming units (CFU-S) relative to the activated population, whereas the activated population contained about 4-fold more day 13 CFU-S on primary transfer relative to the resting population. The two populations produced morphologically distinct splenic colonies; however, the frequency and morphology of in vitro colonies were very similar. Only the resting population provided sufficient stem cells to transfer long-term hematopoietic repopulation to secondary recipient animals after lethal irradiation. On a single cell level, the resting and activated populations exhibited an equivalent ability to differentiate into lymphoid and myeloid progeny. These observations provide further insight into the heterogeneous nature of CFU-S and directly demonstrate that multipotent hematopoietic stem cells are heterogeneous with regard to their clonogenic capacities.
File Format	PDF HTM / HTML
DOI	10.1073/pnas.87.19.7433
Alternate Webpage(s)	http://www.pnas.org/content/87/19/7433.full.pdf
PubMed reference number	1977160
Alternate Webpage(s)	https://doi.org/10.1073/pnas.87.19.7433
Journal	Medline
Volume Number	87
Issue Number	19
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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