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Protein adsorption in static microsystems: effect of the surface to volume ratio.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lionello, Andrea Josserand, J. Kathryn Jensen, Henrik Nymann Girault, Hubert H. |
| Copyright Year | 2005 |
| Abstract | A numerical model for the adsorption kinetics of proteins on the walls of a microchannel has been developed using the finite element method (FEM) to address the coupling with diffusion phenomena in the restricted microchannel volume. Time evolutions of the concentration of one species are given, both in solution and on the microchannel walls. The model illustrates the adsorption limitation sometimes observed when the microdimensions of these systems induce a global depletion of the bulk solution. A new non-dimensional parameter is introduced to predict the final value of the coverage of any microsystem under static adsorption. A working curve and a criteria (h/K[Gamma](max) > 10) are provided in order to choose, for given adsorption characteristics, the value of the volume-to-surface ratio (i.e. the channel height h) avoiding depletion effects on the coverage (relative coverage greater than 90% of the theoretical one). Simulations were compared with confocal microscopy measurements of IgG antibody adsorption on the walls of a PET microchannel. The fit of the model to the experimental data show that the adsorption is under apparent kinetic control. |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/B411179F |
| Alternate Webpage(s) | https://infoscience.epfl.ch/record/59319/files/Labchip_5_2005_254.pdf |
| PubMed reference number | 15726201 |
| Alternate Webpage(s) | https://doi.org/10.1039/B411179F |
| Journal | Medline |
| Volume Number | 5 |
| Issue Number | 3 |
| Journal | Lab on a chip |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
