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Patterns of Simian Virus 40 DNA transcription after acute infection of permissive and nonpermissive cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Khoury, George F. El Byrne, John C. Martin, Malcolm A. |
| Copyright Year | 1972 |
| Abstract | Small amounts of fractionated, denatured, (32)P-labeled DNA from SV40 virus were incubated with a large excess of the complementary RNA of SV40 prepared in vitro with Escherichia coli RNA polymerase; the viral DNA strands were separated on hydroxyapatite columns. The RNA present in green monkey cells late in the lytic cycle reacted with 40-42% of the strand complementary to the in vitro complementary RNA (minus strand), and 60-64% of the opposite (plus) strand. "Early lytic" RNA failed to significantly interact with the plus strand, but formed stable duplex molecules with 35-39% of the minus strand. The RNA prepared from mouse embryo cells 24 hr after infection with SV40 combined with 35-38% of the minus strand and 60-62% of the plus strand. In all cases, the same regions of either the plus or minus strand appear to be transcribed in permissive and nonpermissive infections. |
| File Format | PDF HTM / HTML |
| DOI | 10.1073/pnas.69.7.1925 |
| PubMed reference number | 4340164 |
| Journal | Medline |
| Volume Number | 69 |
| Issue Number | 7 |
| Alternate Webpage(s) | http://www.pnas.org/content/69/7/1925.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1073/pnas.69.7.1925 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
