NDLI: Enzymatic degradation of cellulose from banana stalks for glucose production using cellulolytic activity of Trichoderma viride

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Enzymatic degradation of cellulose from banana stalks for glucose production using cellulolytic activity of Trichoderma viride

Content Provider	Semantic Scholar
Author	Kamara, Dian S. Rachman, Saadah Diana Gaffar, Shabarni Subroto, Toto
Copyright Year	2008
Abstract	Cellulose is the most abundant carbohydrate in nature, but its utilization has not optimized yet. Cellulose consists of glucose monomers linked by �-1,4-glycoside bonds. By hydrolyzing the glycoside bond, we can get the glucose which can be used for various purposes such as bioethanol production. One of problems in hydrolysis of cellulose is the presence of lignin and hemicellulose which make barrier for cellulose hydrolysis. The objective of this research is to investigate the enzymatic degradation efficiency of cellulose from banana stalks by Trichoderma viride. Optimization of cellulose hydrolysis was done by varying initial pH, temperature, particle size, substrate concentration and incubation time. We also determine the effect of substrate pretreatment using sodium hydroxide and delignification using the white rot fungi, Phanerocheate chrysosporium on hydrolysis of cellulose. Pretreatment using sodium hydroxide was done using autoclave for one hour, and pretreatment using white rot fungi was done by incubating the substrate with the fungi for two days. The results of this research showed that the optimum initial pH, temperature, particle size, substrate concentration and incubation time are 5.0, 50°C, 100 mesh, 6% (w/v) and 8 days, respectively. Among the substrate pretreatment done, the sodium hydroxide pretreatment is the best pretreatment resulting the highest glucose concentration.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.chemistry.unpad.ac.id/isc-proceeding/2008/Pdf/PP/0692-0696%20PP101%20Dian%20Siti%20Kamara.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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