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Characterization and Purification of Alkaline Proteases From Viscera of Silver Carp (Hypophthalmichthys molitrix) Fish
| Content Provider | Semantic Scholar |
|---|---|
| Author | Atta, Mohamed B. El-Hamed, Shimaa El-Said Abd Keshk, Samia A. |
| Copyright Year | 2017 |
| Abstract | Proteases from viscera of Silver carp (Hypophthalmichthys molitrix) fish have been extracted and characterized. The alkaline proteases show optimum activity in 0.2M Tris-HCl buffer at pH 8.5 and 45°C using soluble milk casein as substrate. The crude alkaline protease lost about 35% or 51% of its specific activity if it was heated at 50°C or kept at pH 9 for 60 min., respectively. Purification of proteases purified by ammonium sulfate precipitation, gel-filtration using Sephadex G 50 column and ultrafiltration via polyamide membrane (30 KDa) led to increase its specific activity up to 20, 24 and 99 fold, respectively. |
| Starting Page | 40 |
| Ending Page | 54 |
| Page Count | 15 |
| File Format | PDF HTM / HTML |
| DOI | 10.21608/ajas.2017.7572 |
| Volume Number | 48 |
| Alternate Webpage(s) | https://ajas.journals.ekb.eg/article_7572_e8fa8745fe40b440257ba95b466a8709.pdf |
| Alternate Webpage(s) | https://doi.org/10.21608/ajas.2017.7572 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |