NDLI: Corona-Stabilized Interpolyelectrolyte Complexes of SiRNA with Nonimmunogenic, Hydrophilic/Cationic Block Copolymers Prepared by Aqueous RAFT Polymerization †

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Corona-Stabilized Interpolyelectrolyte Complexes of SiRNA with Nonimmunogenic, Hydrophilic/Cationic Block Copolymers Prepared by Aqueous RAFT Polymerization †

Content Provider	Semantic Scholar
Author	Scales, Charles W. Huang, Faqing Vasilieva, Yulia A. Ray, Jacob Convertine, And Anthony J. Mccormick, C. L.
Copyright Year	2006
Abstract	The complexation of small interfering ribonucleic acid (siRNA) with a series of specifically designed block copolymers consisting of the hydrophilic, nonimmunogenic monomer N-(2-hydroxypropyl)methacrylamide (HPMA) and the cationic monomer N-[3-(dimethylamino)propyl]methacrylamide (DMAPMA) has been investigated for potential siRNA stabilization and delivery applications. Specific compositions of poly(HPMA-b-DMAPMA) copolymers were synthesized via aqueous reversible addition−fragmentation chain transfer (RAFT) polymerization and characterized using aqueous size exclusion chromatography with multiangle laser light scattering (SEC-MALLS) and 1H NMR spectroscopy. The degree of soluble complex formation between a model siRNA and the polymers was determined by centrifugal membrane filtration experiments and quantitated by scintillation counting of 32P ATP-labeled siRNA to determine complex solubility and to estimate the degree of complexation relative to cationic and neutral block lengths. Dynamic and static lig.
Starting Page	6871
Ending Page	6881
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1021/ma061453c
Volume Number	39
Alternate Webpage(s)	http://www.lib.gdpu.edu.cn/cyzy/tssjk/lbeyxjzt/2006lbejzt/200906/P020090624522118596430.pdf
Alternate Webpage(s)	https://doi.org/10.1021/ma061453c
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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