NDLI: Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule

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Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsule

Content Provider	Semantic Scholar
Author	Zhang, Xulang Wang, Wei Xie, Yubing Zhang, Ying Wang, Xiuli Ma, Xiaojun
Copyright Year	2006
Abstract	In this study, we investigated the effect of the microenvironment provided by alginate-poly-l-lysine-alginate (APA) microcapsule with liquefied or gelled core on the proliferation, viability, and metabolism of human cells, including anchorage-dependent MCF-7 breast cancer cells and primary fibroblasts, and anchorage-independent K-562 leukemia cells; cells in conventional culture were used as control. The growth pattern of cells in microcapsule was examined by phase-contrast micrography. The cell viability, proliferation, organization, and gene expression were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, hematoxylin and eosin staining, live/dead staining, 5-bromo-20-deoxyuridine labeling, and immunohistochemistry, respectively. Cell metabolism was determined by measuring glucose and lactate concentrations in medium. The results demonstrate that APA microcapsule with liquefied core provides a microenvironment for both anchorage-dependent and anchorage-independent cells to grow into a large cell aggregate and maintain cell viability at a constant level for a period of time. In conclusion, cells in APA microcapsule are alive and have proliferation potential with lower metabolism rate. APA microcapsule may be a useful tool for in vitro tumor cell modeling and anticancer drug screening as well as for cancer gene therapy. In addition, it lays a solid foundation for the use of microencapsulation in cell culture in vitro and cell implantation in vivo.
Starting Page	61
Ending Page	76
Page Count	16
File Format	PDF HTM / HTML
DOI	10.1385/ABAB:134:1:61
PubMed reference number	16891667
Journal	Medline
Volume Number	134
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1385/ABAB:134:1:61
Alternate Webpage(s)	https://doi.org/10.1385/ABAB%3A134%3A1%3A61
Journal	Applied biochemistry and biotechnology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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