NDLI: Estimates of DNA strand breakage in bottlenose dolphin (Tursiops truncatus) leukocytes measured with the Comet and DNA diffusion assays

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Estimates of DNA strand breakage in bottlenose dolphin (Tursiops truncatus) leukocytes measured with the Comet and DNA diffusion assays

Content Provider	Semantic Scholar
Author	Díaz, Adriana Carro, Sandra Santiago, Lívia Maria Estevez, Juan Guevara, Celia Oreja Blanco, Miriam Sánchez, Laima Sánchez, Liena López, Nirka Cruz, Danilo Herrera Breen, James Cuetara, Elizabeth B. Fuentes, Jorge Luis
Copyright Year	2009
Abstract	The analysis of DNA damage by mean of Comet or single cell gel electrophoresis (SCGE) assay has been commonly used to assess genotoxic impact in aquatic animals being able to detect exposure to low concentrations of contaminants in a wide range of species. The aims of this work were 1) to evaluate the usefulness of the Comet to detect DNA strand breakage in dolphin leukocytes, 2) to use the DNA diffusion assay to determine the amount of DNA strand breakage associated with apoptosis or necrosis, and 3) to determine the proportion of DNA strand breakage that was unrelated to apoptosis and necrosis. Significant intra-individual variation was observed in all of the estimates of DNA damage. DNA strand breakage was overestimated because a considerable amount (~29%) of the DNA damage was derived from apoptosis and necrosis. The remaining DNA damage in dolphin leukocytes was caused by factors unrelated to apoptosis and necrosis. These results indicate that the DNA diffusion assay is a complementary tool that can be used together with the Comet assay to assess DNA damage in bottlenose dolphins.
Starting Page	367
Ending Page	372
Page Count	6
File Format	PDF HTM / HTML
PubMed reference number	21637693
DOI	10.1590/s1415-47572009005000030
Journal	Genetics and molecular biology
Volume Number	32
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/d6/7a/gmb-32-2-367.PMC3036937.pdf
Alternate Webpage(s)	http://www.scielo.br/pdf/gmb/v32n2/2008-127.pdf
Alternate Webpage(s)	https://doi.org/10.1590/S1415-47572009005000030
Language	English
Access Restriction	Open
Subject Keyword	Apoptosis Comet Assay DNA Damage DNA-Directed DNA Polymerase Dolphins - mammal Estimated Gel Electrophoresis (lab technique) Leukocytes Mutagens Necrosis Tursiops truncatus peripheral blood
Content Type	Text
Resource Type	Article

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