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Selective Recognition of Cyclic Gmp Using a Fluorescence-based Molecularly Imprinted Polymer
| Content Provider | Semantic Scholar |
|---|---|
| Author | Thanh, Nguyễn Thị Kim Rathbone, Daniel L. Billington, David C. Hartell, Nick A. |
| Copyright Year | 2002 |
| Abstract | Guanosine 3′,5′-cyclic monophosphate (cGMP) plays a role as a second messenger in many different biological systems. Given the ubiquitous nature of cGMP, a simple method of detecting cGMP is of interest. To that end a fluorescent polymer with recognition sites for cGMP has been prepared. Its selectivity and sensitivity were investigated and a dose-dependant decrease in fluorescence of the polymer in the presence of cGMP was observed. In contrast, virtually no effect was detected upon application of the structurally similar molecules, guanosine 5′-monophosphate (GMP) and adenosine 3′,5′-cyclic monophosphate (cAMP), thus demonstrating the high selectivity of this polymer. The association constant for the binding of cGMP to the imprinted polymer was determined in order of 3 × 10 5 M -1. A fluorescent, molecularly imprinted polymer that selectively recognises cGMP may have a useful application as a fluorescent chemosensor for cGMP detection in biological samples. |
| Starting Page | 2499 |
| Ending Page | 2509 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1081/AL-120016540 |
| Volume Number | 35 |
| Alternate Webpage(s) | http://www.ucl.ac.uk/~ucaptt0/AL-120016540.pdf |
| Alternate Webpage(s) | https://doi.org/10.1081/AL-120016540 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
