NDLI: Femtomole mixer for microsecond kinetic studies of protein folding.

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Femtomole mixer for microsecond kinetic studies of protein folding.

Content Provider	Semantic Scholar
Author	Hertzog, David E. Michalet, Xavier Jäger, Marcus Kong, Xiangxu Santiago, Juan G. Weiss, Shimon Bakajin, Olgica
Copyright Year	2004
Abstract	We have developed a microfluidic mixer for studying protein folding and other reactions with a mixing time of 8 mus and sample consumption of femtomoles. This device enables us to access conformational changes under conditions far from equilibrium and at previously inaccessible time scales. In this paper, we discuss the design and optimization of the mixer using modeling of convective diffusion phenomena and a characterization of the mixer performance using microparticle image velocimetry, dye quenching, and Forster resonance energy-transfer (FRET) measurements of single-stranded DNA. We also demonstrate the feasibility of measuring fast protein folding kinetics using FRET with acyl-CoA binding protein.
File Format	PDF HTM / HTML
DOI	10.1021/ac048661s
PubMed reference number	15595857
Journal	Medline
Volume Number	76
Issue Number	24
Alternate Webpage(s)	http://microfluidics.stanford.edu/Publications/Micromixing_EKI/hertzog%20femtomole%20mixer%20for%20kinetic%20protein%20folding%20(AC%202004).pdf
Alternate Webpage(s)	https://web.stanford.edu/group/microfluidics/Publications/Micromixing_EKI/hertzog%20femtomole%20mixer%20for%20kinetic%20protein%20folding%20(AC%202004).pdf
Alternate Webpage(s)	http://www.chem.ucla.edu/~michalet/papers/AC2004.pdf
Alternate Webpage(s)	https://doi.org/10.1021/ac048661s
Journal	Analytical chemistry
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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