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Uso de restritores hídricos na detecção de Sclerotinia sclerotiorum em sementes de feijão, soja e algodão pelo método de incubação em meio Agar-Azul de bromofenol (Neon)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kawasaki, Vivian H. |
| Copyright Year | 2014 |
| Abstract | KAWASAKI, Vivian Hikari. Use of water restriction in the detection of Sclerotinia sclerotiorum in bean, soybeans and cotton seeds by the method of incubation in agar-bromophenol blue (Neon). 2010. 57p. Dissertation (Master in Phytopathology)Universidade Federal de Lavras, Lavras, MG. The importance of crops such as beans, soybeans and cotton is unquestionable due to the significant participation of those in the socio-economic development of Brazil. Among the pathogenic agents causing severe damage in those host species, Sclerotinia sclerotiorum is one the most agressive. The disease, caused by that fungus, known as “white mold” or “Sclerotinia rot”, can be spread by seeds, and by sclerotia which come mixed in commercial lots. These structures are the most effective ways of dissemination of the disease to long distances. Sclerotia are structures of resistance of the fungus that can be viable for over 5 years in soil. The accurate and preventive diagnosis of the pathogen in seeds in the pre-planting and use of healthy seeds before sowing and use of healthy seeds have been some of the main measures to control the disease and are therefore of great importance to control of such disease. In relation to current methods for detection of S. sclerotirorum in seeds of host species, there are some questions, mainly concerning the use of seed germination inhibitors, like 2,4dichlorophenoxyacetic acid, and the schemes of light during the incubation of seeds by the method of agar-bromophenol (Neon). Therefore, the objective of this study was to improve the methodology for detection of Sclerotinia sclerotiorum in bean, soybean and cotton seeds through the use of water restrictors, in the place of 2,4-D, in agar medium containing bromophenol blue and antibiotics (Neon). In the first step, the effects of water retrictors mannitol, sodium chloride and polyethylene glycol (PEG) at differents osmotic potentials were evaluated on the mycelial growth of four Sclerotinia isolates and on the radicle protrusion of seeds of the species under investigation. It was observed that the use of water restrictors at osmotic potentials up to -0.5MPa for PEG and -1.0MPa for mannitol and sodium chloride caused no negative effect on mycelial growth of the isolates tested, and the proved to be satisfactory to inhibit radicle protrusion of beans, soybeans and cotton seeds. Based on the preliminary results, the work focused on comparisons between light regimes and substrates in the detection of S.sclerotiorum in seeds of the three crops. The methodology of semi-selective substrate: solid PDA, bromophenol blue (100 ppm), chloramphenicol (50 ppm) and water restrictor mannitol (1.0MPa) at a temperature of 20°C with incubation in continuous dark was proved to be effective in the detection of that pathogen in routine seeds analysis. * Guidance Committee: José da Cruz Machado UFLA (Major professor), Maria das Graças Guimarães Carvalho – UFLA and Mário Sobral de Abreu UFLA. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://prpg.ufla.br/_ppg/fitopatologia//wp-content/uploads/2015/11/197.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
