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Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dongre, Chaitanya Weerd, Jasper Van Bellini, Nicola Osellame, Roberto Cerullo, Giulio Weeghel, Rob Van Hoekstra, Hugo Pollnau, Markus |
| Copyright Year | 2010 |
| Abstract | We present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of ~200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically. |
| Starting Page | 729 |
| Ending Page | 735 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1364/BOE.1.000729 |
| PubMed reference number | 21258504 |
| Journal | Medline |
| Volume Number | 1 |
| Alternate Webpage(s) | https://ris.utwente.nl/ws/portalfiles/portal/6476034/086-boex1_Dongre.pdf |
| Alternate Webpage(s) | https://doi.org/10.1364/BOE.1.000729 |
| Journal | Biomedical optics express |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
